ASTM E1533-2000(2006) 4-6-二醚基-2-2-苯胺基(DAPI)染色法间接检测细胞培养中菌质的标准实施规程
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【英文标准名称】:StandardPracticeforIndirectDetectionofMycoplasmainCellCultureby4`-6-Diamidino-2-2Phenylindole(DAPI)Staining
【原文标准名称】:4-6-二醚基-2-2-苯胺基(DAPI)染色法间接检测细胞培养中菌质的标准实施规程
【标准号】:ASTME1533-2000(2006)
【标准状态】:现行
【国别】:
【发布日期】:2000
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:医学科学;微生物学;验证
【英文主题词】:DAPIstaining;indicatorcells;mycoplasma
【摘要】:Mycoplasmacontaminationofcellculturesisacommonproblemthatcanaffectthegrowth,metabolism,andfunctionofculturedanimalcells.Theabilitytodetectmycoplasmaincellculturesprovidesanopportunitytoensurethatcellsarefreeofcontamination,andtoreplacethosethatarenot.Foradditionalinformation,seePracticesE1531,E1532,andE1536.Strictadherencetoestablished,well-testedproceduresisnecessary.ThispracticewasdevelopedbyTaskGroupE48.01.02toassistindevelopingandmaintaininganestablishedregimenformycoplasmadetectionbyindirect4x2032;-6-Diamidino-2-Phenylindole(DAPI)fluorochromestaining.Thispracticeisintendedforuseinexaminingculturedanimalcellsforthepresenceofmycoplasmacontamination.Thispracticeisnotintendedforuseinthedetectionofmycoplasmacontaminationinserum,culturemedia,orsystemsotherthanculturesofanimalcells.Allcellculturestobeexaminedformycoplasmashouldundergoaminimumoftwopassagesinantibiotic-freetissueculturemediumbeforetesting.1.1ThispracticecoversproceduresusedforthedetectionofmycoplasmacontaminationbyindirectDNAstaining.1.2ThispracticedoesnotcoverdirectmethodsforthedetectionofmycoplasmaorotherindirectmethodssuchasenzymaticaldetectionorDNAprobes.1.3Thispracticedoesnotcovermethodsfortheidentificationofmycoplasmaorganisms.1.4ThevaluesstatedinSIunitsaretoberegardedasthestandard.Thevaluesgiveninparenthesesareforinformationonly.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C05
【国际标准分类号】:07_100_10
【页数】:3P.;A4
【正文语种】:
【原文标准名称】:4-6-二醚基-2-2-苯胺基(DAPI)染色法间接检测细胞培养中菌质的标准实施规程
【标准号】:ASTME1533-2000(2006)
【标准状态】:现行
【国别】:
【发布日期】:2000
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:医学科学;微生物学;验证
【英文主题词】:DAPIstaining;indicatorcells;mycoplasma
【摘要】:Mycoplasmacontaminationofcellculturesisacommonproblemthatcanaffectthegrowth,metabolism,andfunctionofculturedanimalcells.Theabilitytodetectmycoplasmaincellculturesprovidesanopportunitytoensurethatcellsarefreeofcontamination,andtoreplacethosethatarenot.Foradditionalinformation,seePracticesE1531,E1532,andE1536.Strictadherencetoestablished,well-testedproceduresisnecessary.ThispracticewasdevelopedbyTaskGroupE48.01.02toassistindevelopingandmaintaininganestablishedregimenformycoplasmadetectionbyindirect4x2032;-6-Diamidino-2-Phenylindole(DAPI)fluorochromestaining.Thispracticeisintendedforuseinexaminingculturedanimalcellsforthepresenceofmycoplasmacontamination.Thispracticeisnotintendedforuseinthedetectionofmycoplasmacontaminationinserum,culturemedia,orsystemsotherthanculturesofanimalcells.Allcellculturestobeexaminedformycoplasmashouldundergoaminimumoftwopassagesinantibiotic-freetissueculturemediumbeforetesting.1.1ThispracticecoversproceduresusedforthedetectionofmycoplasmacontaminationbyindirectDNAstaining.1.2ThispracticedoesnotcoverdirectmethodsforthedetectionofmycoplasmaorotherindirectmethodssuchasenzymaticaldetectionorDNAprobes.1.3Thispracticedoesnotcovermethodsfortheidentificationofmycoplasmaorganisms.1.4ThevaluesstatedinSIunitsaretoberegardedasthestandard.Thevaluesgiveninparenthesesareforinformationonly.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C05
【国际标准分类号】:07_100_10
【页数】:3P.;A4
【正文语种】:
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